Product Name: Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb#4322
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 868273-06-7
Product: Org 27569
Specificity Sensitivity: Phospho-Stat5 (Tyr694) (D47E7) XPTM Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.
Species Reactivity: Human, Mouse
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein.
Background: Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24595139?dopt=Abstract
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