L acetate fraction showed the strongest DPPH radical scavenging (0.35 0.094 mg/ml

L acetate fraction showed the strongest DPPH radical scavenging (0.35 0.094 mg/ml) and SOD activities (51.77 four.9 ) whilst the methanol extract showed the highest lowering energy and also the strongest antioxidant activity within the -carotene bleaching assays in comparison to other fractions. The highest phenolic content was found in the ethyl acetate fraction, followed by the crude methanol extract, hexane and water fractions. The outcomes showed a positive correlation involving total phenolic content material with DPPH radical scavenging capacities and SOD activities. The hexane fraction showed potent cytotoxic effect against KB, Ca Ski and HCT 116 cell lines with IC50 of 5.8 0.1 and 9.1 2.0 ug/ml, respectively. The big elements of hexane fraction analysed by GC-MS analysis have been largely methyl esters. Conclusions: The existing study suggests that the methanol extract and ethyl acetate fraction of A. pahangensis can be a potential supply of natural antioxidant for protective as well as prevention of life-threatening ailments. The hexane fraction of A. pahangensis may have the possible to be created into therapeutic alternative for treating cancer. Keywords: Alpinia pahangensis, Antioxidant, Phenolic content material, Cytotoxic activity* Correspondence: [email protected] Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia2013 Phang et al.; licensee BioMed Central Ltd. This can be an Open Access report distributed below the terms from the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is correctly cited.Phang et al. BMC Complementary and Option Medicine 2013, 13:243 http://www.biomedcentral/1472-6882/13/Page 2 ofBackground Alpinia species, from the Zingiberaceae loved ones have already been extensively studied for their chemical and biological properties [1]. Based on the ethnobotanical studies, several species of this genus happen to be employed in conventional medicine, and within the preparations and flavorings of food in several Asian nations. Amongst the Alpinia species, the rhizomes of Alpinia galanga happen to be extensively used as spice and in the remedy of stomachic in China and Thailand; coughs, asthma, bronchitis, headache, inflammation, rheumatoid arthritis and colic in Malaysia [2,3].Icariin Purity 1 wild species, Alpinia mutica has been made use of to treat stomach upset by natives and it has also been reported to show fantastic antioxidant and cytotoxic anticancer properties [4].MPEP Technical Information A handful of novel compounds isolated in the species of Alpinia had been located to possess anti-cancer, antiinflammatory, anti-spasmodic, anti-ulcerogenic, neuroprotective, analgesic, hepatoprotective and cardioprotective properties [5].PMID:24507727 Resulting from its wide therapeutic values, it’s of great interest to conduct much more studies on the unexplored species from this genus which may well possess medicinal properties, yet has not been fully studied. Within this endeavour, Alpinia pahangensis was as a result selected for investigation. Alpinia pahangensis, a wild ginger, occcuring less frequent in the genus, is actually a perennial plant distributed inside the lowlands of Pahang, Malaysia. The rhizomes of A. pahangensis happen to be applied by tribal natives for relieving flatulence. Having said that, there is a restricted study around the biological activity on the A. pahangensis. A recent report by Awang et al. [6] showed that the crucial oil extracted from the rhizomes and leaves of Alpinia pahangensis had very good.