Product Name :
Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)
Applications:
WB
Reactivity :
Mouse IgG(Fcγ Fragment specific)
Conjugate:
HRP
Advantages :
·High lot-to-lot consistency·Increased sensitivity and higher affinity·Animal-free production
Description:
| Description: Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) is designed for detecting the non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3). Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) is based on Monovalent, recombinant single domain antibody to mouse IgG coupled to HRP, and the Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, using Anti-mouse IgG for IP, AlpSdAbs™ VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains. | | Immunogen: Mouse IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: HRP | Specificity: Non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3) | Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light | Background:When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kDa, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP, AlpSdAbs® VHH(HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protein was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protein.{{2765218-56-0} medchemexpress|{2765218-56-0} Purity & Documentation|{2765218-56-0} Purity|{2765218-56-0} custom synthesis} Using Anti-mouse IgG for IP, AlpSdAbs® VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.{{145672-81-7} site|{145672-81-7} Technical Information|{145672-81-7} In Vitro|{145672-81-7} supplier} VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin.PMID:29939573 The size of VHH is extremely small(
Description2 :
Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) is designed for detecting the non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3). Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) is based on Monovalent, recombinant single domain antibody to mouse IgG coupled to HRP, and the Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, using Anti-mouse IgG for IP, AlpSdAbs™ VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.
Immunogen:
Mouse IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Function:
WB: 1:5,000-1:10,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
