Product Name: Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb#9520
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 1069-66-5
Product: Valproic acid (sodium salt)
Specificity Sensitivity: Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb detects endogenous levels of Smad3 when phosphorylated at Ser423/425. This antibody does not cross-react with other family members.
Species Reactivity: Human, Mouse, Rat
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser423/425 of Smad3.
Background: Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Sm
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25228009?dopt=Abstract
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