Product Name: Phospho-A-Raf (Ser299) Antibody#4431
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 122795-43-1
Product: Gadodiamide (hydrate)
Specificity Sensitivity: Phospho-A-Raf (Ser299) Antibody detects endogenous levels of A-Raf only when phosphorylated at serine 299. The antibody does not cross-react with B-Raf or Raf-1 under physiological conditions.
Species Reactivity: Human, Mouse, Rat
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser299 of human A-Raf. Antibodies are purified by protein A and peptide affinity chromatography.
Background: A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Th
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24548894?dopt=Abstract
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