Product Name: Phospho-Threonine-X-Arginine Antibody#2351
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry: 1:800
Peptide ELISA: 1:1000
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 124-94-7
Product: Triamcinolone
Specificity Sensitivity: Phospho-Threonine-X-Arginine Antibody detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. This antibody detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for Lys compared to Arg
Species Reactivity: Human, Mouse, Rat, Monkey, All Species Expected
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the phospho-Thr-X-Arg motif. Antibodies are purified by protein A and peptide affinity chromatography.
Background: Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the –
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18199551?dopt=Abstract
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