Product Name: TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred)#8184
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 41332-24-5
Product: NP118809
Specificity Sensitivity: TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) recognizes endogenous levels of total TNF-α protein. TNF-α (D5G9) Rabbit mAb #6945 is more sensitive by western blot.
Species Reactivity: Human
Source Purification: Monoclonal antibody is produced by immunizing animals with recombinant human TNF-α protein.
Background: TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TN
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24316970?dopt=Abstract
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