Product Name: Phospho-Rpb1 CTD (Thr4) Antibody#14934
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 14919-77-8
Product: Benserazide (hydrochloride)
Specificity Sensitivity: Phospho-Rpb1 (Thr4) Antibody recognizes endogenous levels of Rpb1 protein only when the carboxy-terminal domain (CTD) heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at Thr4. This antibody does not cross-react with Rpb1 CT
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr4 of human Rpb1 CTD heptapeptide repeat. Antibodies are purified by protein A and peptide affinity chromatography.
Background: RNA polymerase II (RNAPII) is a large multi-protein complex that functions as a DNA-dependent RNA polymerase, catalyzing the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (1). The largest subunit, RNAPII subunit B
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26244545?dopt=Abstract
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