Product Name: Phospho-Rpb1 CTD (Ser2/5) Antibody#4735
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry: 1:25
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 219793-45-0
Product: BTB06584
Specificity Sensitivity: Phospho-Rpb1 CTD (Ser2/5) Antibody detects endogenous levels of Rpb1 when the carboxy-termial domain heptapeptide repeat [Tyr1,Ser2,Pro3,Thr4,Ser5,Pro6,Ser7] is dually phosphorylated at Ser2 and Ser5 and singly phosphorylated at either Ser2 or Ser5.
Species Reactivity: Human, Mouse, Rat
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide. Antibodies are purified by protein A and peptide affinity chromatography.
Background: RNA polymerase II (RNAPII) is a large multi-protein complex that functions as a DNA-dependent RNA polymerase, catalyzing the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (1). The largest subunit, RNAPII subunit B
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26307093?dopt=Abstract
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