Product Name: Non-phospho-Src (Tyr527) Antibody#2107
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 125314-64-9
Product: Ro 31-8220
Specificity Sensitivity: Non-phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when dephosphorylated at tyrosine 527. It may also cross-react with Yes, Fyn and Slk based on sequence similarity.
Species Reactivity: Human, Mouse, Rat
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr530 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Background: The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, bu
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21498541?dopt=Abstract
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