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ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx COX-1 site transporter) and SLC16A10 (which codes for an L-DOPA efflux transporter). From the complete set of data (n = six, two control samples, two samples at 24 h post-infection and two samples at 60 h post infection), we could extract Expression values for 11 out of 14 genes of interest. We then utilized the Pearson’s correlation test to evaluate the co-expression hyperlinks in between these genes and ACE2. We found that eight key genes involved within the metabolism of dopamine and/or trace amines exhibited statistically substantial co-expression links with ACE2 across all experimental conditions. Of note, essentially the most robust correlation link was observed for MAOB, followed by SLC7A9 and SULT1A1 (Table three).Int. J. Mol. Sci. 2021, 22,tern. Furthermore anticipated, the L-DOPA efflux transporters SLC3A2 and SLC7A8 have been detected in the basolateral membrane of enterocytes. A low and diffuse staining pattern was observed for SLC16A10. Finally, no TH staining could be detected (Figure S1), in accordance with genomics analyses. According to these mined information, a scheme summarizing the predicted dopamine/trace amines metabolic pathways Bcl-B review taking spot in human enterocytes 6 of 16 is shown in Figure 2.Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking spot in human Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking location in huenterocytes of of smaller intestine. This scheme is based on the mining of human expression atlases and on previously man enterocytesthe the small intestine. This scheme is primarily based onthe mining of human expression atlases and on previously publishedbiochemical and/or functional data obtained in intestinal or non-intestinal cells. The molecules integrated within this published biochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules incorporated in this scheme comprise: angiotensin-converting enzyme (ACE2), solute carrier family members 6 member 19 (SLC6A19), solute carrier scheme comprise: angiotensin-converting enzyme two 2 (ACE2), solute carrier family six member 19 (SLC6A19), solute carrier loved ones 33member 11(SLC3A1), solute carrier loved ones 77member 99(SLC7A9), dopa-decarboxylase (DDC), sulfotransferase family members member (SLC3A1), solute carrier household member (SLC7A9), dopa-decarboxylase (DDC), sulfotransferase family members 1A member 11 (SULT1A1),sulfotransferase family 1A member 22 (SULT1A2),sulfotransferase household 1A member 33 family members 1A member (SULT1A1), sulfotransferase loved ones 1A member (SULT1A2), sulfotransferase household 1A member (SULT1A3), cytochrome P450 family members two subfamily D member six (CYP2D6), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carrier family 3 member two (SLC3A2), solute carrier family members 7 member 8 (SLC7A8) and solute carrier loved ones 6 member 10 (SLC16A10). Table three. Correlation analysis of ACE2 mRNA levels with crucial genes in the dopamine/trace amines metabolic pathways in SARS-CoV2-infected human enterocytes. DDC 0.84 0.035 MAOA 0.86 0.025 MAOB 0.96 0.001 SULT1A1 0.92 0.007 SLC7A9 0.95 0.003 SLC3A1 0.87 0.02 SLC6A19 0.88 0.017 SLC3A2 0.9 0.Expression data have been extracted from Lamers et al. [34] as well as the Pearson’s test was applied to assess correlation coefficient (r, upper line) and statistical significance (p-value, reduced line)) among ACE2 and genes of interest. Gene symbols: dopa-decarboxylase (DDC), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carr

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Author: Interleukin Related