ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes

ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes for an L-DOPA efflux transporter). In the whole set of information (n = six, two control samples, two samples at 24 h post-infection and two samples at 60 h post infection), we could extract expression values for 11 out of 14 genes of interest. We then applied the Bcr-Abl Storage & Stability Pearson’s correlation test to evaluate the co-expression links involving these genes and ACE2. We located that eight important genes involved in the metabolism of dopamine and/or trace amines exhibited statistically significant co-expression hyperlinks with ACE2 across all experimental circumstances. Of note, probably the most robust correlation hyperlink was observed for MAOB, followed by SLC7A9 and SULT1A1 (Table 3).Int. J. Mol. Sci. 2021, 22,tern. Moreover anticipated, the L-DOPA efflux transporters SLC3A2 and SLC7A8 had been detected in the basolateral membrane of enterocytes. A low and diffuse staining pattern was observed for SLC16A10. Ultimately, no TH staining might be detected (Figure S1), in accordance with genomics analyses. Depending on these mined information, a scheme summarizing the predicted dopamine/trace amines metabolic pathways taking spot in human enterocytes 6 of 16 is shown in Figure two.Figure two. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking spot in human Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking location in huenterocytes of of compact intestine. This scheme is determined by the mining of human expression atlases and on previously man enterocytesthe the modest intestine. This scheme is based onthe mining of human expression atlases and on previously publishedbiochemical and/or functional data obtained in intestinal or non-intestinal cells. The molecules integrated in this published biochemical and/or functional data obtained in intestinal or non-intestinal cells. The molecules included in this scheme comprise: angiotensin-converting enzyme (ACE2), solute carrier family six JAK Synonyms member 19 (SLC6A19), solute carrier scheme comprise: angiotensin-converting enzyme 2 2 (ACE2), solute carrier family members 6 member 19 (SLC6A19), solute carrier family members 33member 11(SLC3A1), solute carrier family members 77member 99(SLC7A9), dopa-decarboxylase (DDC), sulfotransferase household member (SLC3A1), solute carrier family member (SLC7A9), dopa-decarboxylase (DDC), sulfotransferase household 1A member 11 (SULT1A1),sulfotransferase loved ones 1A member 22 (SULT1A2),sulfotransferase family members 1A member 33 family members 1A member (SULT1A1), sulfotransferase loved ones 1A member (SULT1A2), sulfotransferase family 1A member (SULT1A3), cytochrome P450 family members two subfamily D member six (CYP2D6), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carrier family 3 member 2 (SLC3A2), solute carrier household 7 member 8 (SLC7A8) and solute carrier household six member 10 (SLC16A10). Table 3. Correlation analysis of ACE2 mRNA levels with key genes from the dopamine/trace amines metabolic pathways in SARS-CoV2-infected human enterocytes. DDC 0.84 0.035 MAOA 0.86 0.025 MAOB 0.96 0.001 SULT1A1 0.92 0.007 SLC7A9 0.95 0.003 SLC3A1 0.87 0.02 SLC6A19 0.88 0.017 SLC3A2 0.9 0.Expression information were extracted from Lamers et al. [34] as well as the Pearson’s test was applied to assess correlation coefficient (r, upper line) and statistical significance (p-value, reduce line)) amongst ACE2 and genes of interest. Gene symbols: dopa-decarboxylase (DDC), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carr