Product Name: NEDD8 Antibody#2745
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:25
Immunohistochemistry: 1:200
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 1133432-46-8
Product: GDC-0834 (Racemate)
Specificity Sensitivity: This antibody detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO1, SUMO2, SUMO3 and ISG15.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human NEDD8 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background: Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family (1,2). The covalent attachment of NEDD8 to target proteins,
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19804774?dopt=Abstract
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