This data was confirmed by western blotting and double immunofluorescence staining

persed in the matrix. This data corroborated the SEM results, which showed no granular particles in the middle or in the surface of the inserts. Representative photomicrographs of retinas showing the smaller number of RCG in control glaucomatous rats treated or not with placebo inserts and the beneficial effects of DIZE inserts in these cells; n = 5 per group. (B) Quantification of RGC in retinas of rats; n = 5 per group. p<0.05 vs. control (CTRL) and #p<0.05 vs. glaucoma (GLAU) (One-way ANOVA followed by the Newman-Keuls post test). CTRL: control non-treated group; CTRL+P+I: control group that received placebo inserts; CTRL+D+I: control group that received DIZE inserts; GLAU: glaucoma non-treated group; GLAU+P+I: glaucoma group that received placebo inserts; and GLAU+D+I: glaucoma group that received DIZE inserts.Note the optic nerve head cupping in control glaucomatous animals treated or not with placebo inserts (P+I) when compared to all other groups; n = 5 per group. Treatment with D+I was able to reverse this effect. In detail are representative photomicrographs of longitudinal sections of the optic nerve. A large reduction in the neural fibers was observed in control glaucomatous rats treated or not with P+I. CTRL: control non-treated group; CTRL+P+I: control group that received placebo inserts; CTRL+D+I: control group that received DIZE inserts; GLAU: glaucoma non-treated group; MedChemExpress BCTC pubmed ID:http://www.ncbi.nlm.nih.gov/pubmed/19667219 GLAU+P+I: glaucoma group that received placebo inserts; and GLAU+D+I: glaucoma group that received DIZE inserts. In vivo drug biodistribution. (A) Representative images after 30 min, 2, 4, 6 and 18 hours of administration PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19666821 of the radiolabeled formulations; n = 5 per group. Note that 99mTc-DIZE eye drops began to clear from the corneal regio